An accurate and non-labor intensive method for the determination of syringyl to guaiacyl ratio in lignin.
Identifieur interne : 003710 ( Main/Exploration ); précédent : 003709; suivant : 003711An accurate and non-labor intensive method for the determination of syringyl to guaiacyl ratio in lignin.
Auteurs : Mark Govender [Afrique du Sud] ; Tamara Bush ; Andrew Spark ; Samar K. Bose ; Raymond C. FrancisSource :
- Bioresource technology [ 1873-2976 ] ; 2009.
Descripteurs français
- KwdFr :
- Acide chlorhydrique (composition chimique), Biotechnologie (méthodes), Bois (composition chimique), Dioxanes (composition chimique), Eau (composition chimique), Eucalyptus (métabolisme), Lignine (composition chimique), Modèles chimiques (MeSH), Modèles statistiques (MeSH), Méthode en double aveugle (MeSH), Nitrobenzènes (composition chimique), Oxygène (composition chimique), Populus (MeSH), Reproductibilité des résultats (MeSH).
- MESH :
- composition chimique : Acide chlorhydrique, Bois, Dioxanes, Eau, Lignine, Nitrobenzènes, Oxygène.
- métabolisme : Eucalyptus.
- méthodes : Biotechnologie.
- Modèles chimiques, Modèles statistiques, Méthode en double aveugle, Populus, Reproductibilité des résultats.
English descriptors
- KwdEn :
- Biotechnology (methods), Dioxanes (chemistry), Double-Blind Method (MeSH), Eucalyptus (metabolism), Hydrochloric Acid (chemistry), Lignin (chemistry), Models, Chemical (MeSH), Models, Statistical (MeSH), Nitrobenzenes (chemistry), Oxygen (chemistry), Populus (MeSH), Reproducibility of Results (MeSH), Water (chemistry), Wood (chemistry).
- MESH :
- chemical , chemistry : Dioxanes, Hydrochloric Acid, Lignin, Nitrobenzenes, Oxygen, Water.
- chemistry : Wood.
- metabolism : Eucalyptus.
- methods : Biotechnology.
- Double-Blind Method, Models, Chemical, Models, Statistical, Populus, Reproducibility of Results.
Abstract
The syringyl to guaiacyl (S:G) ratio of hardwood lignin has long been identified as a significant parameter in delignification processes and more recent results have shown that it is also important in determining the amount of ethanol that can be obtained from fermentation of hydrolyzed wood. Acidolysis of Klason or acid insoluble lignin in dioxane/water/HCl was being investigated when syringyl and guaiacyl nuclei with a diketone-containing sidechain were observed as the major products. The area ratio of the two gas chromatogram peaks appeared to be indicative of the S:G ratio. After optimization of the method the relative standard deviation was found to be in the range of 0.3-3.76% for Klason lignin from a wide range of Eucalyptus grandis grown in South Africa. The method was then compared to nitrobenzene oxidation (NBO) using 13 poplars in a double-blind study. The respective S:G ratios were used to calculate percentages of S units and when these values were plotted against each other a linear correlation was obtained with a slope of approximately 1.0 (R(2)=0.86). The largest discrepancy for any poplar was 6.9% (62% vs. 58% S units). Both methods convincingly demonstrated a significant decrease in lignin content with an increase in the S:G ratio. Discussion is presented on a series of reaction that could lead to the formation of the two diketones.
DOI: 10.1016/j.biortech.2009.06.009
PubMed: 19576762
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Box 17001, Congella 4013, Durban, South Africa.</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Council for Science and Industrial Research and University of KwaZulu-Natal, Forestry and Forest Product Research Centre, P.O. Box 17001, Congella 4013, Durban</wicri:regionArea><wicri:noRegion>Durban</wicri:noRegion></affiliation></author><author><name sortKey="Bush, Tamara" sort="Bush, Tamara" uniqKey="Bush T" first="Tamara" last="Bush">Tamara Bush</name></author><author><name sortKey="Spark, Andrew" sort="Spark, Andrew" uniqKey="Spark A" first="Andrew" last="Spark">Andrew Spark</name></author><author><name sortKey="Bose, Samar K" sort="Bose, Samar K" uniqKey="Bose S" first="Samar K" last="Bose">Samar K. Bose</name></author><author><name sortKey="Francis, Raymond C" sort="Francis, Raymond C" uniqKey="Francis R" first="Raymond C" last="Francis">Raymond C. Francis</name></author></analytic><series><title level="j">Bioresource technology</title><idno type="eISSN">1873-2976</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biotechnology (methods)</term><term>Dioxanes (chemistry)</term><term>Double-Blind Method (MeSH)</term><term>Eucalyptus (metabolism)</term><term>Hydrochloric Acid (chemistry)</term><term>Lignin (chemistry)</term><term>Models, Chemical (MeSH)</term><term>Models, Statistical (MeSH)</term><term>Nitrobenzenes (chemistry)</term><term>Oxygen (chemistry)</term><term>Populus (MeSH)</term><term>Reproducibility of Results (MeSH)</term><term>Water (chemistry)</term><term>Wood (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide chlorhydrique (composition chimique)</term><term>Biotechnologie (méthodes)</term><term>Bois (composition chimique)</term><term>Dioxanes (composition chimique)</term><term>Eau (composition chimique)</term><term>Eucalyptus (métabolisme)</term><term>Lignine (composition chimique)</term><term>Modèles chimiques (MeSH)</term><term>Modèles statistiques (MeSH)</term><term>Méthode en double aveugle (MeSH)</term><term>Nitrobenzènes (composition chimique)</term><term>Oxygène (composition chimique)</term><term>Populus (MeSH)</term><term>Reproductibilité des résultats (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Dioxanes</term><term>Hydrochloric Acid</term><term>Lignin</term><term>Nitrobenzenes</term><term>Oxygen</term><term>Water</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Wood</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Acide chlorhydrique</term><term>Bois</term><term>Dioxanes</term><term>Eau</term><term>Lignine</term><term>Nitrobenzènes</term><term>Oxygène</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Eucalyptus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biotechnology</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Eucalyptus</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Biotechnologie</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Double-Blind Method</term><term>Models, Chemical</term><term>Models, Statistical</term><term>Populus</term><term>Reproducibility of Results</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Modèles chimiques</term><term>Modèles statistiques</term><term>Méthode en double aveugle</term><term>Populus</term><term>Reproductibilité des résultats</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The syringyl to guaiacyl (S:G) ratio of hardwood lignin has long been identified as a significant parameter in delignification processes and more recent results have shown that it is also important in determining the amount of ethanol that can be obtained from fermentation of hydrolyzed wood. Acidolysis of Klason or acid insoluble lignin in dioxane/water/HCl was being investigated when syringyl and guaiacyl nuclei with a diketone-containing sidechain were observed as the major products. The area ratio of the two gas chromatogram peaks appeared to be indicative of the S:G ratio. After optimization of the method the relative standard deviation was found to be in the range of 0.3-3.76% for Klason lignin from a wide range of Eucalyptus grandis grown in South Africa. The method was then compared to nitrobenzene oxidation (NBO) using 13 poplars in a double-blind study. The respective S:G ratios were used to calculate percentages of S units and when these values were plotted against each other a linear correlation was obtained with a slope of approximately 1.0 (R(2)=0.86). The largest discrepancy for any poplar was 6.9% (62% vs. 58% S units). Both methods convincingly demonstrated a significant decrease in lignin content with an increase in the S:G ratio. Discussion is presented on a series of reaction that could lead to the formation of the two diketones.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19576762</PMID><DateCompleted><Year>2009</Year><Month>12</Month><Day>03</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2976</ISSN><JournalIssue CitedMedium="Internet"><Volume>100</Volume><Issue>23</Issue><PubDate><Year>2009</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Bioresource technology</Title><ISOAbbreviation>Bioresour Technol</ISOAbbreviation></Journal><ArticleTitle>An accurate and non-labor intensive method for the determination of syringyl to guaiacyl ratio in lignin.</ArticleTitle><Pagination><MedlinePgn>5834-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.biortech.2009.06.009</ELocationID><Abstract><AbstractText>The syringyl to guaiacyl (S:G) ratio of hardwood lignin has long been identified as a significant parameter in delignification processes and more recent results have shown that it is also important in determining the amount of ethanol that can be obtained from fermentation of hydrolyzed wood. Acidolysis of Klason or acid insoluble lignin in dioxane/water/HCl was being investigated when syringyl and guaiacyl nuclei with a diketone-containing sidechain were observed as the major products. The area ratio of the two gas chromatogram peaks appeared to be indicative of the S:G ratio. After optimization of the method the relative standard deviation was found to be in the range of 0.3-3.76% for Klason lignin from a wide range of Eucalyptus grandis grown in South Africa. The method was then compared to nitrobenzene oxidation (NBO) using 13 poplars in a double-blind study. The respective S:G ratios were used to calculate percentages of S units and when these values were plotted against each other a linear correlation was obtained with a slope of approximately 1.0 (R(2)=0.86). The largest discrepancy for any poplar was 6.9% (62% vs. 58% S units). Both methods convincingly demonstrated a significant decrease in lignin content with an increase in the S:G ratio. Discussion is presented on a series of reaction that could lead to the formation of the two diketones.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Govender</LastName><ForeName>Mark</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Council for Science and Industrial Research and University of KwaZulu-Natal, Forestry and Forest Product Research Centre, P.O. Box 17001, Congella 4013, Durban, South Africa.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bush</LastName><ForeName>Tamara</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Spark</LastName><ForeName>Andrew</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Bose</LastName><ForeName>Samar K</ForeName><Initials>SK</Initials></Author><Author ValidYN="Y"><LastName>Francis</LastName><ForeName>Raymond C</ForeName><Initials>RC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>07</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Bioresour 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MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>11</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2009</Year><Month>04</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>06</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>7</Month><Day>7</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>7</Month><Day>7</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19576762</ArticleId><ArticleId IdType="pii">S0960-8524(09)00667-1</ArticleId><ArticleId IdType="doi">10.1016/j.biortech.2009.06.009</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Afrique du Sud</li></country></list><tree><noCountry><name sortKey="Bose, Samar K" sort="Bose, Samar K" uniqKey="Bose S" first="Samar K" last="Bose">Samar K. Bose</name><name sortKey="Bush, Tamara" sort="Bush, Tamara" uniqKey="Bush T" first="Tamara" last="Bush">Tamara Bush</name><name sortKey="Francis, Raymond C" sort="Francis, Raymond C" uniqKey="Francis R" first="Raymond C" last="Francis">Raymond C. Francis</name><name sortKey="Spark, Andrew" sort="Spark, Andrew" uniqKey="Spark A" first="Andrew" last="Spark">Andrew Spark</name></noCountry><country name="Afrique du Sud"><noRegion><name sortKey="Govender, Mark" sort="Govender, Mark" uniqKey="Govender M" first="Mark" last="Govender">Mark Govender</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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